WASHINGTON — Rohit Bhargava, an IIT-Delhi graduate and now with the University of Illinois at Urbana-Champaign, has developed stainless staining that provides a new tool for clinicians and researchers for better diagnosis of cancer.
Till date, prepared biopsy samples were stained and examined under a light microscope.
Using infrared spectroscopic imaging for microscopy, Bhargava has reported the development of label-free chemical imaging to provide the same information as molecular stains.
Instead of using stains, the new method measures the chemical constitution of cells and tissues directly.
The outcome is that that molecular stains can be reproduced without staining the tissue but by using the intrinsic molecular contrast of the tissue and computation.
The study relies on computation instead of staining to provide biopsy images.
“Infrared and optical imaging seemed to be distinct modes for getting important data in pathology. This study shows a close link between the two, allowing the user to choose the best method to address their needs,” the Indian American scientist commented in a university statement.
Thus, any sample can be stained for desired stains without material cost, time or effort while leaving precious tissue pristine for downstream analyses.